Three-Dimensional Electron Microscopy of Macromolecular Assemblies
Visualization of Biological Molecules in Their Native State
ISBN13: 9780195182187ISBN10: 0195182189
Paperback,
432 pages
Also available:
Hardback
Jan 2006,
In Stock
Price:
$105.00 (06)Description
In the past ten years there has been a tremendous surge in applications of cryo-electron microscopy in the study of macromolecular assemblies, in especially those in single-particle form. Although the path toward atomic resolution is much more tedious than in electron crystallography of two-dimensional crystals, realization of such resolution for entirely asymmetrical molecules is now in close reach.In Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in Their Native State, Joachim Frank describes the mathematical principles and working methods of single-particle reconstruction--a method designed to retrieve structural information from electron micrographs showing many "copies" of molecules trapped in ice in random orientations. The technique is uniquely suited to obtain three-dimensional images of molecular machines in different functional states, as it does not require crystals. This revised edition includes a basic introduction to transmission electron microscopy and the design of the instruments, and it covers specimen preparation in greater depth. Frank addresses the remarkable convergence of processing methods that has taken place, making it possible to sharpen the focus of some sections. New sections have been added that cover segmentation as well as various techniques of docking and fitting. Finally, appendix material is now included; this tabulates applications and methods of single-particle reconstruction and describes the most important mathematical tools and theorems of Fourier processing.
Product Details
432 pages; 128 halftones & line illus.; 6-1/8 x 9-1/4; ISBN13: 978-0-19-518218-7ISBN10: 0-19-518218-9About the Author(s)
Joachim Frank is an Investigator at the Howard Hughes Medical Institute. He is also Professor of Biomedical Sciences and Adjunct Professor of Biology at the State University of New York at Albany, Adjunct Professor of Biochemistry and Molecular Biophysics at Columbia University, Research Professor of Cell Biology at New York State University Medical School, and head of the Laboratory of Computational Biology and Macromolecular Imaging at Health Research, Inc., the Wadsworth Center.
