Description

Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes.

Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology.

This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.

Reviews

"Timely and well organized, flow cytometry technology as described here will have a powerful impact on how one approaches high-throughput technologies. This first scholarly treatment of the subject should be read by flow cytometry experts, academic researchers, students, as well as those who are developing new biotechnology."--Mark J. Soloski, Department of Medicine, The Johns Hopkins University School of Medicine

Product Details

400 pages; 20 halftones, 87 line illus.; 6-1/8 x 9-1/4; ISBN13: 978-0-19-515234-0ISBN10: 0-19-515234-4

About the Author(s)