A Lineweaver-Burk plot is used to analyse kinetic data on enzyme-catalysed reactions. The reciprocal of the rate of formation of products (1/v) is plotted against the reciprocal of the substrate concentration (1/[S]). The equation is

Where [S] and K_M have units of µmol L^-1. v and v_max have units of µmol L^-1 s^-1.The maximum velocity v_max of the enzymolysis is the reciprocal of the y-intercept of the best fit line. The slope of the best fit line is the Michaelis constant K_M divided by v_max.

To calculate v_max and K_M enter data pairs (S, v) and then click the Least-Squares Analysis tab. To see the Lineweaver-Burk plot click the Plot tab. To pick off a value on the graph, move your cursor to the desired location then click and hold.