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Bromham: Reading the Story in DNA
Chapter 4
Endless copies
Or: how do I amplify DNA?
Heroes of the genetic revolution – Francis Crick
The Wellcome Trust holds the archive of Crick’s papers, accessible from:
http://genome.wellcome.ac.uk
TechBox 4.2 - DNA amplification
Examples of protocols and guides to primer design can be found at:
Protocol-Online http://www.protocol-online.org/prot/Molecular_Biology/PCR/
TechBox 4.3- Primer design
There is a range of primer design software available, both licensed and freeware. For example, Primer3:
http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi
Case study 4.1 Glorious mud: using DNAreassociation kinetics to quantify biodiversity of soil bacteria
For a beginners guide to metagenomics see: www.bioteach.ubc.ca/Biodiversity/Metagenomics/