Case study 06

Fungal resistance in rice

Your institute has been contracted to enhance the antifungal properties of New Plant Type rice. It is envisaged that a broad spectrum of resistance could be obtained by using constructs containing chitinase, glucanase and RIP genes. You are required to produce for your project leader a research strategy for obtaining these constructs and introducing them either singly or in various combinations into rice. The contents of the report are at your discretion but it should include a description of the properties and source of the genes to be used, the means by which the constructs are to be introduced into the vectors, a detailed description of the vectors and the transformation systems to be used and how the constructs are to be tested. Unless directly relevant to specific gene isolation or analytical procedures, it is not necessary to include details of standard molecular or tissue culture techniques. The proposal should be fully risk assessed. Some analysis of the world-wide problem of fungal infections of rice should also be included.

Oxford University Press, Online Resource Centre, Case study 06.

Case study 06