Atkins & de Paula: |
||||
| Atkins & De Paula: Physical Chemistry for the Life Sciences | LIVING GRAPHS |
|||
| Living graphs homepage | Determination of fluorescence lifetime 13.52 |
|||
From measurements of the lifetime of fluorescent decay in the presence of a quencher, we can determine the individual rate constants from the intercept and slope of this graph. The formula is
Where Q is the quencher concentration (mol L-1), kq has units of L mol-1s-1, and kf has units of s-1. To plot |
||||
Copyright © Oxford University Press 2005. All rights reserved. Legal Statement. |
||||