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Atkins & de Paula:
Physical Chemistry for the Life Sciences

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Atkins & De Paula: Physical Chemistry for the Life Sciences
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Determination of fluorescence lifetime 13.52

From measurements of the lifetime of fluorescent decay in the presence of a quencher, we can determine the individual rate constants from the intercept and slope of this graph.

The formula is

Where Q is the quencher concentration (mol L-1), kq has units of L mol-1s-1, and kf has units of s-1.

To plot enter the parameters (i.e., kq and kf) and hit New Plot. Up to 5 plots can be displayed at one time. The Clear button will remove all plots. To see the parameters for each plot hit the Legend on/off button. The Redraw button will refresh the graph. This is useful when the function domain (i.e., Q ) has been changed. To see the value of each plot at a given point, move your cursor to the desired location then click and hold.

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