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Which of the following statements about column chromatography is not correct?

a) Affinity chromatography involves the attachment to the column matrix of groups or molecules known that specifically bind to the wanted protein.

b) In reverse phase chromatography the wanted protein can be selectively eluted by solutions of different hydrophobicities or ionic strengths.

c) Ion-exchange chromatography involves the use of different ionic groups attached to the column matrix that specifically bind to the wanted protein.

d) Gel-filtration chromatography separates proteins on their ability to bind to specific groups on the column matrix.

Which of the following statements about SDS polyacrylamide gel electrophoresis is correct?

a) Wanted proteins can be tested for their biological activity after separation by SDS polyacrylamide gel electrophoresis.

b) Proteins are solubilized but not denatured when separated by SDS polyacrylamide gel electrophoresis.

c) SDS polyacrylamide gel electrophoresis separates proteins on the basis of charge.

d) SDS polyacrylamide gel electrophoresis separates proteins on the basis of size.

Which of the following statements about isoeletric focusing is correct?

a) Proteins separated by isoelectric focusing cannot be tested for biological activity.

b) Proteins separated by isoelectric focusing can be tested for biological activity.

c) The separation of proteins by isoelectric focusing is only based on charge.

d) The separation of proteins by isoelectric focusing is only based on size.

Which of the following statements about the use of mass spectrometry in protein investigation is not correct?

a) Mass spectrometry involves ionized molecules in the gas phase.

b) Mass spectrometry is used for analysing the sequence of peptides.

c) Ionic fragments are separated according to their mass-to-charge ratio in Mass spectrometry.

d) Mass spectrometry involves the separation of ionic fragments on a gel.

Which of the following statements about Western Blotting is correct?

a) The detection of a particular protein by Western Blotting relies on the very specific interaction between the protein and its antibody.

b) The detection of a particular protein by Western Blotting relies on labelling the protein with a specific dye.

c) The detection of a particular protein by Western Blotting relies on labelling the antibody with a specific dye.

d) The detection of a particular protein by Western Blotting relies on the denaturation of the protein.

Oxford University Press, Online Resource Centre, Chapter 05.