Immunity: Chapter 06, B Cells and Humoral Immunity

Table of Contents

1. 6-1 Structure of Antibodies
2. 6-2 Structural Properties of Antibodies
3. 6-3 Effector Functions of Antibodies
4. 6-4 Monoclonal Antibodies
5. 6-5 Antibody-Based Methods: Radioimmunoassay and Hemagglutination
6. 6-6 Antibody-Based Methods: ELISA, Immunoblotting and FACS
7. 6-7 Antigen Receptors of B Lymphocytes
8. 6-8 B Cell Antigen Receptor Signaling and B Cell Coreceptors
9. 6-9 Early and T Cell-Independent Antibody Responses by B Cells
10. 6-10 Initiation of T Cell-Dependent Antibody Responses
11. 6-11 Hypermutation and Isotype Switching in the Germinal Center
12. 6-12 Selection and Differentiation of B Cells in the Germinal Center

6-5 Antibody-Based Methods: Radioimmunoassay and Hemagglutination

Work through the following questions, checking all the correct answers. For each question there may be more than one correct answer.

1

Which of the following statements are true? a) A large insoluble immune precipitate occurs when the antigen is in considerable excess over the antibody. b) The epitope is the site on an antigen that is bound by a particular antibody molecule. c) The radioimmunoassay (RIA) can be used to measure the amount of an antigen of interest, such as a hormone in the blood. d) In the radioimmunoassay, the antigen bound to antibodies is frequently recovered by attaching the antibody to latex beads. e) Immune complexes that form inside an individual may cause tissue damage and disease. f) In the hemagglutination test, antibodies are used to aggregate red blood cells. g) Protein A, which binds to antibodies and is useful for immunoprecipitation, is a protein made by the pathogenic bacterium Staphylococcus aureus. h) An immune precipitate requires an antigen to contain at least two epitopes reactive with the antibodies being used. CorrectWrong: b, c, d, e, f, g, and h are true.Your answer has been saved.